Forsyth S E, Hoger A, Hoger J H
Department of Bioengineering, University of California at San Diego, La Jolla 92093, USA.
FEBS Lett. 1997 Jun 9;409(2):277-82. doi: 10.1016/s0014-5793(97)00514-0.
A 5.1 kb cDNA encoding an inward rectifier K+ channel (BIK) was isolated from a bovine aortic endothelial cell library. The cDNA codes for a 427-amino-acid protein with two putative transmembrane regions. Sequence analysis reveals that BIK is a member of the Kir2.1 family of inward rectifier K+ channels. Expression in Xenopus oocytes showed that BIK is a K+-specific strong inward rectifier channel that is sensitive to extracellular Ba2+, Cs+, and a variety of anti-arrhythmic agents. Northern analysis revealed that endothelial cells express a 5.5 kb BIK mRNA that is sensitive to shear stress.
从牛主动脉内皮细胞文库中分离出一个编码内向整流钾通道（BIK）的5.1 kb cDNA。该cDNA编码一个含有两个假定跨膜区域的427个氨基酸的蛋白质。序列分析表明，BIK是内向整流钾通道Kir2.1家族的成员。在非洲爪蟾卵母细胞中的表达表明，BIK是一种对细胞外Ba2+、Cs+和多种抗心律失常药物敏感的钾特异性强内向整流通道。Northern分析显示，内皮细胞表达一种对剪切应力敏感的5.5 kb BIK mRNA。
