Li X, De Boer S H, Ward L J
Agriculture and Agri-Food Canada, Pacific Agriculture Research Centre, Vancouver, BC, Canada.
Lett Appl Microbiol. 1997 Jun;24(6):431-4. doi: 10.1046/j.1472-765x.1997.00283.x.
An oligonucleotide probe was selected from the 16S rRNA gene of Clavibacter michiganensis subsp. sepedonicus for specific in situ hybridization. The rhodamine-labelled oligonucleotide probe was used in conjunction with an indirect immunofluorescence procedure based on a specific monoclonal antibody detected with a fluorescein-labelled conjugate. Simultaneous labelling of bacterial cells with the oligonucleotide and antibody probes allows accurate microscopic identification of single cells when isolation and other methods of confirming bacterial identity are not possible.
从密执安棒杆菌亚种马铃薯环腐病菌的16S rRNA基因中选择一个寡核苷酸探针用于特异性原位杂交。罗丹明标记的寡核苷酸探针与基于用荧光素标记的偶联物检测的特异性单克隆抗体的间接免疫荧光程序结合使用。当无法进行分离和其他确认细菌身份的方法时，用寡核苷酸和抗体探针同时标记细菌细胞可实现单细胞的准确显微镜鉴定。
