Wasano N, Saitoh H, Ohba M
Institute of Biological Control, Kyushu University, Fukuoka, Japan.
Lett Appl Microbiol. 1997 Jun;24(6):438-40. doi: 10.1046/j.1472-765x.1997.00056.x.
The 130 kDa parasporal inclusion proteins of the Lepidoptera-specific Bacillus thuringiensis reference strains for four H serovars were examined for sequences of 14 N-terminal amino acids. The four sequences fell into a single category, MNRNNQNEYEVIDA, and were dissimilar to any of the established sequences for Lepidoptera- and/or Diptera-killing crystal proteins. They were highly related to the reported sequence of the 130 kDa protein of the strain Buibui (serovar japonensis), which is specific for scarabaeid coleopterans.
对4种H血清型的鳞翅目特异性苏云金芽孢杆菌参考菌株的130 kDa伴孢晶体蛋白进行了14个N端氨基酸序列检测。这4个序列属于同一类别，即MNRNNQNEYEVIDA，与任何已确定的鳞翅目和/或双翅目杀虫晶体蛋白序列均不相同。它们与报道的Buibui菌株（血清型日本亚种）130 kDa蛋白序列高度相关，该菌株对金龟子科甲虫具有特异性。
