Maloney S E, Marks T S, Sharp R J
Centre for Applied Microbiology and Research, Salisbury, Wilts, UK.
Lett Appl Microbiol. 1997 Jun;24(6):441-4. doi: 10.1046/j.1472-765x.1997.00151.x.
Thermophilic (75 degrees C), anaerobic biodegradation of chlorobenzoates was investigated using different inocula from geothermal and non-geothermal environments. Microbial dehalogenation of 3-chlorobenzoate (0.5 mmol l-1 was achieved by two mixed cultures growing anaerobically at 75 degrees C. One culture consisted of a facultative anaerobe and two obligate anaerobes, one of which was a methanogen, isolated from terrestrial sediments from hot springs in New Zealand. The other culture, derived from a non-geothermal environment, consisted of a Clostridium spp. and a non-spore-forming obligate anaerobe. No degradation of either 2-chlorobenzoate or 4-chlorobenzoate was achieved by these thermophilic cultures over the same time period. This is the first reported biotransformation of this chlorinated aromatic at a temperature of 75 degrees C.
利用来自地热和非地热环境的不同接种物，研究了嗜热菌（75摄氏度）对氯苯甲酸酯的厌氧生物降解。两种混合培养物在75摄氏度厌氧条件下生长，实现了对3-氯苯甲酸酯（0.5 mmol l-1）的微生物脱卤。一种培养物由兼性厌氧菌和两种专性厌氧菌组成，其中一种是产甲烷菌，从新西兰温泉的陆地沉积物中分离得到。另一种培养物来自非地热环境，由一种梭菌属细菌和一种不形成芽孢的专性厌氧菌组成。在相同时间段内，这些嗜热培养物未实现对2-氯苯甲酸酯或4-氯苯甲酸酯的降解。这是首次报道在75摄氏度温度下对这种氯代芳烃的生物转化。
