Miyake J, Hara M
National Institute for Advanced Interdisciplinary Research, AIST/MITI, Ibaraki, Japan.
Adv Biophys. 1997;34:109-26. doi: 10.1016/s0065-227x(97)89635-9.
Methods of constructing proteins were examined with special reference to the molecular assembly using photosynthetic RCs as membrane proteins. Molecular assemblies at the interfaces were studied by LB films, adsorption to the surface and reconstitution into liposomes and bilayer lipid membranes. The applications of biological specific ligands (recognition and binding), combinatorial chemical method, 2-D and 3-D order array assemblies and modification of protein molecules to make fusion proteins, as well as physical methods of manipulation of molecules by AFM tips and electric fields were reviewed.
研究了构建蛋白质的方法，特别提及了以光合反应中心作为膜蛋白的分子组装。通过LB膜、表面吸附以及重组到脂质体和双层脂质膜中，研究了界面处的分子组装。综述了生物特异性配体（识别与结合）、组合化学方法、二维和三维有序阵列组装以及蛋白质分子修饰以制备融合蛋白的应用，以及通过原子力显微镜探针和电场操纵分子的物理方法。
