来自澳大利亚绿树蛙（Litoria xanthomera）皮肤腺体的新型凯林抗菌肽。第2部分。使用质谱及相关技术进行序列测定。
New caerin antibacterial peptides from the skin glands of the Australian tree frog Litoria xanthomera. Part 2. Sequence determination using mass spectrometry and associated techniques.
作者信息
Steinborner S T, Waugh R J, Bowie J H, Tyler M J
机构信息
Department of Chemistry, University of Adelaide, Australia.
出版信息
Rapid Commun Mass Spectrom. 1997;11(9):997-1000. doi: 10.1002/(SICI)1097-0231(19970615)11:9<997::AID-RCM942>3.0.CO;2-2.
Mass spectrometric sequencing, enzymic digestion and Edman degradation provide the structures of the two antimicrobial peptides from the skin glands of the Australian tree frog Litoria xanthomera as:- Gly Leu Phe Ser Val Leu Gly Ala Val Ala Lys His Val Leu Pro His Val Val Pro Val Ile Ala Glu Lys Leu (NH2) (caerin 1.6), and Gly Leu Phe Lys Val Leu Gly Ser Val Ala Lys His Leu Leu Pro His Val Ala Pro Val Ile Ala Glu Lys Leu (NH2) (caerin 1.7).
质谱测序、酶切和埃德曼降解确定了澳大利亚树蛙黄纹树蛙皮肤腺体中两种抗菌肽的结构如下：- 甘氨酸-亮氨酸-苯丙氨酸-丝氨酸-缬氨酸-亮氨酸-甘氨酸-丙氨酸-缬氨酸-丙氨酸-赖氨酸-组氨酸-缬氨酸-亮氨酸-脯氨酸-组氨酸-缬氨酸-缬氨酸-脯氨酸-缬氨酸-异亮氨酸-丙氨酸-谷氨酸-赖氨酸-亮氨酸（NH2）（caerin 1.6），以及甘氨酸-亮氨酸-苯丙氨酸-赖氨酸-缬氨酸-亮氨酸-甘氨酸-丝氨酸-缬氨酸-丙氨酸-赖氨酸-组氨酸-亮氨酸-亮氨酸-脯氨酸-组氨酸-缬氨酸-丙氨酸-脯氨酸-缬氨酸-异亮氨酸-丙氨酸-谷氨酸-赖氨酸-亮氨酸（NH2）（caerin 1.7）。
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