Mahbouba M, Smith H J
Adv Exp Med Biol. 1977;86A:247-60. doi: 10.1007/978-1-4684-3282-4_15.
Macromolecules have been prepared containing native insulin carried by a modified insulin skeleton made by partially thiolating the insulin hexamer and forming intermolecular cross-links through disulphide bridges. Oxidation of partially thiolated insulin (0.5-0.7 SH group/mole), formed by reacting insulin with AHTL, with, (a) potassium ferricyanide, (b) Cu++-oxygen gave water soluble macromolecules containing 20-26 and 410-708 monomer units respectively which had rod-random coil shape (light scattering). The larger molecules formed by (b) contained 8g-atom CU++/hexamer unit and insulin. The insulin was firmly bound within the marcomolecules and was probably bound within an insulin-modified insulin hexamer through coordination to copper.
已经制备了大分子，其包含由修饰的胰岛素骨架携带的天然胰岛素，该修饰的胰岛素骨架通过部分硫醇化胰岛素六聚体并通过二硫键形成分子间交联而制成。通过使胰岛素与AHTL反应形成的部分硫醇化胰岛素（0.5 - 0.7个巯基/摩尔）与（a）铁氰化钾、（b）Cu²⁺ - 氧气氧化，分别得到含有20 - 26个和410 - 708个单体单元的水溶性大分子，其具有棒状 - 无规卷曲形状（光散射）。由（b）形成的较大分子含有8克原子Cu²⁺/六聚体单元和胰岛素。胰岛素牢固地结合在大分子内，并且可能通过与铜配位而结合在胰岛素修饰的胰岛素六聚体内。
