Jiang X, Ma Y, Yang S
Institute of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin.
Zhonghua Zhong Liu Za Zhi. 1996 Mar;18(2):105-8.
Human bone marrow mononuclear cells were cultured on a layer of stromal cells derived from bone marrow of a patient with esophageal cancer. After five-week cultivation, the mononuclear cells could grow independently of stromal cells in liquid culture and had been passaged over 50 generations with a doubling time of 48 hours. The cell line thus established is called B3HM. The growth of B3HM appeared anchorage independent in semi-solid culture and produced 423 +/- 87.7 colonies per 5 X 10(4) cells. Immunofluorescence analysis showed that the cells were CD19+, CD20+, CD13+, CD15+, with both B cell and granulocyte surface markers. The cells or cell homogenate could induce leukemia when inoculated in nude mice and "615" inbred mice. The induced mouse leukemia was transplantable and L3T4 positive in more than 80% of the leukemic cells. The pathogenesis of the induced mouse leukemia is worthy of further investigation.
人骨髓单个核细胞在取自一名食管癌患者骨髓的基质细胞层上进行培养。培养五周后，单个核细胞能够在液体培养中独立于基质细胞生长，并且已经传代50多次，倍增时间为48小时。由此建立的细胞系称为B3HM。B3HM在半固体培养中呈现出不依赖贴壁生长的特性，每5×10(4)个细胞可产生423±87.7个集落。免疫荧光分析表明，这些细胞CD19+、CD20+、CD13+、CD15+，同时具有B细胞和粒细胞表面标志物。将这些细胞或细胞匀浆接种到裸鼠和“615”近交系小鼠中可诱发白血病。诱发的小鼠白血病具有可移植性，并且在超过80%的白血病细胞中L3T4呈阳性。诱发的小鼠白血病的发病机制值得进一步研究。
