van Leusden M R, Kuikman I, Sonnenberg A
Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam.
Biochem Biophys Res Commun. 1997 Jun 27;235(3):826-30. doi: 10.1006/bbrc.1997.6892.
A novel cytoplasmic splice variant of the human beta4 integrin subunit has been identified by reverse transcription polymerase chain reaction using mRNA from cultured keratinocytes as the template. This fifth beta4 variant, called beta4E, is expressed in a wide variety of tissues including the epidermis, lung, duodenum, heart, spleen and stomach and in several human epithelial cell lines. The beta4E cDNA contains an insert of 37 base pairs which produces a frame shift in the sequence encoding the beta4 cytoplasmic domain and generates a new stop codon after a stretch of cDNA encoding a unique 114-amino acid peptide. Analysis of the genomic organization at the site of this insertion in the human beta4 gene reveals that beta4E is produced by partial retention of an intron in the final transcript.
利用培养的角质形成细胞的mRNA作为模板，通过逆转录聚合酶链反应，已鉴定出一种新型的人β4整合素亚基的细胞质剪接变体。这种第五种β4变体称为β4E，在多种组织中表达，包括表皮、肺、十二指肠、心脏、脾脏和胃，以及几种人类上皮细胞系。β4E cDNA包含一个37个碱基对的插入片段，该片段在编码β4细胞质结构域的序列中产生移码，并在一段编码独特的114个氨基酸肽的cDNA延伸后产生一个新的终止密码子。对人β4基因中该插入位点的基因组结构分析表明，β4E是由最终转录本中一个内含子的部分保留产生的。
