Delage R, Roy J, Jacques L, Bernier V, Delâge J M, Darveau A
Centre d'Hématologie et d'Immunologie Clinique, Hôpital du St-Sacrement, Laval University, Quebec, Ste-Foy, Canada.
Br J Haematol. 1997 Jun;97(3):589-95. doi: 10.1046/j.1365-2141.1997.852725.x.
Persistent polyclonal B-cell lymphocytosis is a benign lymphoproliferative disorder of unknown aetiology occurring exclusively in women, characterized by typical binucleated lymphocytes, polyclonal expansion of B cells and elevated serum IgM. Owing to the role of Bcl-2 oncogene in inhibition of apoptosis, we have investigated the presence of the bcl-2/Ig gene rearrangement. Bcl-2/Ig gene rearrangement was determined by polymerase chain reaction targeting the usual breakpoint regions of the t(14;18). Bcl-2/Ig gene rearrangement was identified in all six patients and, more importantly, multiple rearrangements were present in five patients. The frequency of the bcl-2/Ig gene rearrangement is estimated to be of one translocation in 1 x 10(2) to 1 x 10(3) peripheral blood mononuclear cells. We conclude that persistent polyclonal B-cell lymphocytosis is associated with bcl-2/Ig gene rearrangement. These findings are of clinical importance because these patients may be misdiagnosed as having a leukaemic expression of non-Hodgkin's lymphoma.
持续性多克隆B细胞淋巴细胞增多症是一种病因不明的良性淋巴细胞增殖性疾病，仅发生于女性，其特征为典型的双核淋巴细胞、B细胞多克隆扩增以及血清IgM升高。由于Bcl-2癌基因在抑制细胞凋亡中的作用，我们研究了bcl-2/Ig基因重排的存在情况。通过针对t(14;18)常见断点区域的聚合酶链反应来确定bcl-2/Ig基因重排。在所有6例患者中均鉴定出bcl-2/Ig基因重排，更重要的是，5例患者存在多种重排。bcl-2/Ig基因重排的频率估计为每1×10(2)至1×10(3)个外周血单个核细胞中有一次易位。我们得出结论，持续性多克隆B细胞淋巴细胞增多症与bcl-2/Ig基因重排有关。这些发现具有临床重要性，因为这些患者可能被误诊为患有非霍奇金淋巴瘤的白血病样表现。
