[肝移植后慢性排斥反应中的闭塞性动脉病。通过原位杂交检测巨细胞病毒基因组及通过免疫组织化学检测病毒抗原]
[Obliterative arteriopathy in chronic rejection after hepatic transplantation. Search of cytomegalovirus genome by in situ hybridization and viral antigens by immunohistochemistry].
作者信息
Martin L, Sin A, Kantelip B, Mougin C, Heyd B, Miguet J P, Mantion G, Bresson-Hadni S, Lab M, Carbillet J P
机构信息
Laboratoire d'Anatomie et Cytologie Pathologiques, CHU Jean-Minjoz, Besançon.
出版信息
Gastroenterol Clin Biol. 1997;21(4):259-64.
OBJECTIVES
This study was performed to assess the role of cytomegalovirus in the parietal infection of intrahepatic arteries in the pathogenesis of obliterative arteriopathy from chronic rejection after orthotopic liver transplantation.
METHODS
We studied two groups of liver transplants by in situ hybridization and immunohistochemistry: group 1, including 10 liver grafts with obliterating arteriopathy, and group 2 including 7 liver grafts without any arterial disorders. The results were correlated with clinical data (cytomegalovirus infection and acute rejection).
RESULTS
By in situ hybridization, cytomegalovirus DNA was identified in the media in 70% of transplants in group 1 and 42% in group 2. Detection of immediate early and late antigens by immunohistochemistry was negative. Cytomegalovirus infections were often associated with acute rejection.
CONCLUSION
These results suggest that cytomegalovirus detected in arteries is latent, and that cytomegalovirus probably does not play a role in the pathogenesis of chronic rejection obliterative arteriopathy.
目的
本研究旨在评估巨细胞病毒在原位肝移植后慢性排斥反应所致闭塞性动脉病发病机制中对肝内动脉壁感染的作用。
方法
我们通过原位杂交和免疫组织化学研究了两组肝移植：第1组包括10例有闭塞性动脉病的肝移植物，第2组包括7例无任何动脉病变的肝移植物。结果与临床数据（巨细胞病毒感染和急性排斥反应）相关。
结果
通过原位杂交，在第1组70%的移植物和第2组42%的移植物的中膜中鉴定出巨细胞病毒DNA。免疫组织化学检测即刻早期和晚期抗原均为阴性。巨细胞病毒感染常与急性排斥反应相关。
结论
这些结果表明，在动脉中检测到的巨细胞病毒是潜伏性的，并且巨细胞病毒可能在慢性排斥反应闭塞性动脉病的发病机制中不起作用。
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