大菱鲆（Scophthalmus maximus L.）卵巢在卵母细胞成熟过程中体外合成17,20β,21-三羟基-4-孕烯-3-酮。

In vitro synthesis of 17,20 beta,21-trihydroxy-4-pregnen-3-one by ovaries of turbot (Scophthalmus maximus L.) during oocyte maturation.

作者信息

Mugnier C, Gaignon J L, Fostier A

机构信息

NATA, Noirmoutier, France.

出版信息

Gen Comp Endocrinol. 1997 Jul;107(1):63-73. doi: 10.1006/gcen.1997.6895.

DOI:10.1006/gcen.1997.6895

PMID:9208306

Abstract

Ovaries from female turbot (Scophthalmus maximus L.), a serial spawner, were incubated in vitro with 17-hydroxy[1,2,6,7-3H]progesterone or [7-3H(N)]pregnenolone (P5-3H) during the spawning season. Several metabolites comigrated on TLC and HPLC with known reference steroids and were identified after chemical reaction and crystallization. Incubation with P5-3H generated many 4-ene steroids, accounting for 55% of total radioactivity, indicating strong 3 beta-HSD activity. The major steroids produced by ovaries were testosterone, androstenedione, and 17,21-dihydroxy-4-pregnene-3,20-dione. In addition, 17,20 alpha-dihydroxy-4-pregnen-3-one was also identified, while small quantities of 17,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-S) (maximum 1.7% of the total radioactivity) were also synthesized. The identity of 20 beta-S was confirmed in incubates with nonlabeled 17-hydroxyprogesterone by mass spectrometry. Production of 17,20 beta-dihydroxy-4-pregnen-3-one was not apparent.

摘要

在产卵季节，将连续产卵的雌性大菱鲆（Scophthalmus maximus L.）的卵巢与17-羟基[1,2,6,7-³H]孕酮或[7-³H(N)]孕烯醇酮（P5-³H）进行体外孵育。几种代谢产物在薄层色谱（TLC）和高效液相色谱（HPLC）上与已知的参考类固醇共迁移，并在化学反应和结晶后得以鉴定。用P5-³H孵育产生了许多4-烯类固醇，占总放射性的55%，表明具有很强的3β-羟基类固醇脱氢酶（3β-HSD）活性。卵巢产生的主要类固醇为睾酮、雄烯二酮和17,21-二羟基-4-孕烯-3,20-二酮。此外，还鉴定出了17,20α-二羟基-4-孕烯-3-酮，同时也合成了少量的17,20β,21-三羟基-4-孕烯-3-酮（20β-S）（最高占总放射性的1.7%）。通过质谱法在与未标记的17-羟基孕酮的孵育物中证实了20β-S的身份。17,20β-二羟基-4-孕烯-3-酮的产生并不明显。