Peng S, Dong J, Yang J
Institute of Toxicology and Pharmacology Academy of Military Medical Sciences, Beijing.
Zhonghua Yu Fang Yi Xue Za Zhi. 1996 May;30(3):141-3.
Toxic action of mycotoxin T-2 and its metabolite T-2 tetraol and deepoxy T-2; tetraol was studied by cytotoxicity and animal toxicity tests to explore the relationship between toxin T-2 and its structure. Restults showed that proliferation of LLC-PK1 cells and synthesis of DNA could be inhibited by both toxin T-2 and T-2 tetraol. Toxicity of toxin T-2 was 100 times greater than that of T-2 tetraol. There was no obvious toxicity to the proliferation of LLC-PK1 cells and synthesis of DNA in a dose of 10 mg/L deepoxy T-2 tetraol. But, toxin T-2 caused obvious damage in heart muscle and articular cartilage of chicken embryos, and T-2 tetraol and deepoxy T-2 tetraol in heart muscle, but not in articular cartilage. T-2 tetraol produced by hydrolysis of toxin T-2 had toxicity to certain extent, but its strength was significantly less than that of the latter. It suggests that the epoxide group in toxin T-2 played a determinant role for its toxicity. If epoxy cycle of the basic nucleus of the molecule is open, its toxicity will change significantly.
通过细胞毒性和动物毒性试验研究了霉菌毒素T-2及其代谢产物T-2四醇和脱环氧T-2；四醇的毒性作用，以探讨毒素T-2与其结构之间的关系。结果表明，毒素T-2和T-2四醇均可抑制LLC-PK1细胞的增殖和DNA合成。毒素T-2的毒性比T-2四醇大100倍。10mg/L的脱环氧T-2四醇对LLC-PK1细胞的增殖和DNA合成无明显毒性。但是，毒素T-2对鸡胚心肌和关节软骨造成明显损伤，T-2四醇和脱环氧T-2四醇对心肌有损伤，但对关节软骨无损伤。毒素T-2水解产生的T-2四醇有一定毒性，但其强度明显小于后者。这表明毒素T-2中的环氧基团对其毒性起决定性作用。如果分子基本核的环氧环打开，其毒性将发生显著变化。
