Li Y, Fu Z, Chen X, Han R
Institute of Materia Medica, CAMS and PUMC, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1996 Apr;18(2):111-5.
Experiments were conducted by using scrape-loading and dye transfer (SLDT) method to study the gap junction intercellular communication (GJIC) of Chinese hamster lung cells (V79), mouse fibrous cells (Balb/c-3T3), rat liver cells (WB) and human embryonal lung cells (2BS). We also observed the inhibition of the GJIC by TPA and the antagonistic effect of Curcumin derivatives on TPA. The results indicated that V79, WB, 3T3 and 2BS normal cells showed medium level of GJIC, and TPA could inhibit the GJIC to some extents. Curcumin derivatives (91022, 91022-S) could counteract the inhibition of TPA-induced GJIC. It was also found that human lung adenocarcinoma cell (A549) and GLC lacked GJIC, and 91022 could improve the GJIC of A549 cell. It may be related to its anticancer activity.
采用刮除加载和染料转移（SLDT）方法进行实验，以研究中国仓鼠肺细胞（V79）、小鼠成纤维细胞（Balb/c - 3T3）、大鼠肝细胞（WB）和人胚肺细胞（2BS）的间隙连接细胞间通讯（GJIC）。我们还观察了佛波酯（TPA）对GJIC的抑制作用以及姜黄素衍生物对TPA的拮抗作用。结果表明，V79、WB、3T3和2BS正常细胞表现出中等水平的GJIC，TPA可在一定程度上抑制GJIC。姜黄素衍生物（91022、91022 - S）可抵消TPA诱导的GJIC抑制作用。还发现人肺腺癌细胞（A549）和GLC缺乏GJIC，而91022可改善A549细胞的GJIC。这可能与其抗癌活性有关。
