Colleoni G W, Silva M R, Silva R S, Costa F F, Kerbauy J, Saad S T
Divisão de Hematologia, Universidade Federal de São Paulo, Brazil.
Acta Oncol. 1997;36(3):313-5. doi: 10.3109/02841869709001269.
The aim of the present study was to characterize the type of BCR-ABL transcript and to correlate the molecular feature with bone marrow histology. For this purpose, we analysed the BCR-ABL rearrangement in 26 patients with chronic myeloid leukemia (CML) in the chronic phase by RT-PCR, and we also classified the bone marrow histology according to the predominance of granulocylic (GRAN) or granulocytic and megakaryocytic (GRAM/MEG) proliferation, after analysis of two independent observers. We did not find any significant difference in survival of patients presenting b2-a2 and b3-a2 transcripts or GRAN and GRAN/MEG bone marrow types, nor did we find any significant correlation of the type of BCR-ABL transcript with the bone marrow histological subgroups GRAN and GRAN-MEG (Fisher's test = 0.31). Thus, we conclude that the presence of exon b3 is not correlated to bone marrow histology in CML.
本研究的目的是鉴定BCR-ABL转录本的类型，并将分子特征与骨髓组织学相关联。为此，我们通过逆转录聚合酶链反应（RT-PCR）分析了26例慢性期慢性髓性白血病（CML）患者的BCR-ABL重排情况，并且在两名独立观察者分析之后，我们还根据粒细胞（GRAN）或粒细胞和巨核细胞（GRAM/MEG）增殖的优势对骨髓组织学进行了分类。我们未发现呈现b2-a2和b3-a2转录本的患者或GRAN和GRAN/MEG骨髓类型患者的生存率存在任何显著差异，也未发现BCR-ABL转录本类型与骨髓组织学亚组GRAN和GRAN-MEG之间存在任何显著相关性（Fisher检验=0.31）。因此，我们得出结论，外显子b3的存在与CML的骨髓组织学无关。
