Nakagawa Y, Lee Y M, Lehmberg E, Herrmann R, Herrmann R, Moskowitz H, Jones A D, Hammock B D
Department of Entomology, University of California, Davis 95616, USA.
Eur J Biochem. 1997 Jun 1;246(2):496-501. doi: 10.1111/j.1432-1033.1997.t01-1-00496.x.
An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose < 1.8 microg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiurus quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).
一种昆虫选择性蝎毒素（AaIT5）从北非蝎子黄肥尾蝎的毒液中纯化得到，其氨基酸序列通过自动Edman降解、电喷雾电离质谱和序列比对相结合的方法确定。这种昆虫毒素对烟草夜蛾具有很强的毒性（100%致死剂量<1.8微克/100毫克体重），并表现出明显的昆虫特异性和多种症状。皮下注射后对小鼠无毒。该毒素的分子量为6882道尔顿，其氨基酸序列与黄肥尾蝎抗昆虫毒素4（AaIT4）、以色列金蝎抑制性抗昆虫毒素（LqhIT2、LqqIT2）以及犹大杀牛蝎抑制性抗昆虫毒素（BjIT2）相似。
