Kai K, Mitsuno K, Tanaka A, Oka K, Jinno A, Goto N, Ami Y, Andoh S
Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
Leukemia. 1997 Apr;11 Suppl 3:236-8.
PVC111, PVC211, PVC321 and PVC441 cause neurological disorders associated with tremor and paralysis in rats. We tested the pathogenicity of these viruses in mice. Although histopathological studies revealed spongiform degeneration in the spinal cords of NFS mice infected with each PVC virus, only PVC441 caused a high incidence of tremor and paralysis. Further studies with PVC441 revealed dose and age dependence for tremor induction. In contrast to NFS mice, BALB/c, DBA/2 and C57BL/6 mice infected with PVC441 virus showed no neurological symptoms, although the virus replicated in each strain to titers within 5-fold of the titer in NFS mice. Despite absence of neurological symptoms, high degree of neuronal degeneration in the lumbar spinal cord was found in PVC441-infected BALB/c mice. Low degree of neuronal degeneration was found in PVC441-infected DBA/2 or C57BL/6 mice. Genetic crosses of these resistant mice with susceptible NFS mice indicated that resistance to PVC441-induced tremor induction was dominant in all strains and suggested that various host genes may control the susceptibility of mice to tremor induction by PVC441 virus. Sequencing of env-LTR region of PVC441 revealed an intermediate character between PVC211 and F-MuLV.
PVC111、PVC211、PVC321和PVC441可导致大鼠出现与震颤和麻痹相关的神经紊乱。我们在小鼠中测试了这些病毒的致病性。尽管组织病理学研究显示，感染每种PVC病毒的NFS小鼠脊髓出现海绵状变性，但只有PVC441导致了高发生率的震颤和麻痹。对PVC441的进一步研究揭示了震颤诱导的剂量和年龄依赖性。与NFS小鼠不同，感染PVC441病毒的BALB/c、DBA/2和C57BL/6小鼠未表现出神经症状，尽管该病毒在每个品系中复制的滴度与在NFS小鼠中的滴度相差不超过5倍。尽管没有神经症状，但在感染PVC441的BALB/c小鼠的腰脊髓中发现了高度的神经元变性。在感染PVC441的DBA/2或C57BL/6小鼠中发现了低度的神经元变性。这些抗性小鼠与易感的NFS小鼠进行遗传杂交表明，对PVC441诱导的震颤的抗性在所有品系中都是显性的，并表明各种宿主基因可能控制小鼠对PVC441病毒诱导震颤的易感性。对PVC441的env-LTR区域进行测序，发现其具有介于PVC211和F-MuLV之间的中间特征。
