Osada H, Grutz G G, Axelson H, Forster A, Rabbitts T H
Laboratory of Molecular Biology, Medical Research Council, Cambridge, United Kingdom.
Leukemia. 1997 Apr;11 Suppl 3:307-12.
The LIM-only protein Lmo2, originally identified as an oncogenic protein in human T cell leukemia, is essential for erythropoiesis. A possible role for Lmo2 in transcription during erythropoiesis has been investigated. Direct interaction of Lmo2 was observed in vitro and in vivo with the zinc finger transcription factor GATA-1, as well as with the basic helix-loop-helix (bHLH) transcription factor Tall. By using mammalian two-hybrid analysis, E47/Tall/Lmo2/GATA-1 protein complex could be demonstrated. Thus, a molecular link exists between three proteins crucial for erythropoiesis. This data suggest that variations in amounts of complexes involving Lmo2, Tall, and GATA-1 could be important for erythroid differentiation.
仅含LIM结构域的蛋白Lmo2最初被鉴定为人类T细胞白血病中的一种致癌蛋白，它对红细胞生成至关重要。人们已经研究了Lmo2在红细胞生成过程中转录中的可能作用。在体外和体内均观察到Lmo2与锌指转录因子GATA-1以及碱性螺旋-环-螺旋（bHLH）转录因子Tall直接相互作用。通过哺乳动物双杂交分析，可证明E47/Tall/Lmo2/GATA-1蛋白复合物的存在。因此，在对红细胞生成至关重要的三种蛋白之间存在分子联系。这些数据表明，涉及Lmo2、Tall和GATA-1的复合物数量变化可能对红系分化很重要。
