聚（乙二醇）衍生物与马来酸酐梳状共聚物对L-天冬酰胺酶的化学修饰
Chemical modification of L-asparaginase with comb-shaped copolymer of poly(ethylene glycol) derivative and maleic anhydride.
作者信息
Saito T, Ueno T, Sekine T, Kodera Y, Hiroto M, Matsushima A, Nishimura H, Inada Y
机构信息
Department of Materials Science and Technology, Toin University of Yokohama, Japan.
出版信息
Leukemia. 1997 Apr;11 Suppl 3:408-9.
L-asparaginase from Escherichia coli, an antitumor enzyme, was chemically modified with a comb-shaped copolymer of poly(ethylene glycol) derivative and maleic anhydride (activated PM). The PM-modified asparaginase lost the immunoreactivity with retaining high enzymic activity and also prolonged the clearance time in blood. Intraperitoneal administration of PM-asparaginase markedly increased the mean survival-time of lymphoma L5178Y-bearing mice in comparison with that of unmodified asparaginase. Pretreatment of mice with PM-asparaginase before immunizing with unmodified asparaginase extremely suppressed the anti-asparaginase antibody production.
来自大肠杆菌的L-天冬酰胺酶是一种抗肿瘤酶，用聚乙二醇衍生物与马来酸酐的梳状共聚物（活化的PM）对其进行化学修饰。经PM修饰的天冬酰胺酶失去了免疫反应性，但保留了高酶活性，并且还延长了在血液中的清除时间。与未修饰的天冬酰胺酶相比，腹腔注射PM-天冬酰胺酶显著延长了荷淋巴瘤L5178Y小鼠的平均存活时间。在用未修饰的天冬酰胺酶免疫之前，用PM-天冬酰胺酶对小鼠进行预处理可极大地抑制抗天冬酰胺酶抗体的产生。
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