人蔗糖酶-异麦芽糖酶（SI）基因的葡萄糖依赖性转录调控。
Glucose-dependent transcriptional regulation of the human sucrase-isomaltase (SI) gene.
作者信息
Rodolosse A, Carrière V, Chantret I, Lacasa M, Zweibaum A, Rousset M
机构信息
Unité de Recherches sur la Différenciation Cellulaire Intestinale, INSERM U178, Villejuif, France.
出版信息
Biochimie. 1997 Feb-Mar;79(2-3):119-23. doi: 10.1016/s0300-9084(97)81502-7.
We have previously shown that the transcription of the human sucrase-isomaltase (SI) gene was negatively regulated by glucose. Using two clonal metabolic variants of the human colon adenocarcinoma cell line Caco-2 we demonstrate here that: 1) although similar growth-related variations of phosphoenolpyruvate carboxykinase (PEPCK), frutose 1,6-diphosphatase (F1, 6-dPase), pyruvate kinase (PK) and SI mRNA levels are observed, only F1,6-dPase, PK and SI mRNA levels vary in the same way in response to modifications of glucose utilization; and 2) regulatory elements responsible for the glucose-dependent transcription of the SI gene are located within the -370/+30 region of the promoter.
我们之前已经表明，人蔗糖酶-异麦芽糖酶（SI）基因的转录受到葡萄糖的负调控。利用人结肠腺癌细胞系Caco-2的两种克隆代谢变体，我们在此证明：1）尽管观察到磷酸烯醇丙酮酸羧激酶（PEPCK）、果糖1,6-二磷酸酶（F1,6-dPase）、丙酮酸激酶（PK）和SI mRNA水平有类似的与生长相关的变化，但只有F1,6-dPase、PK和SI mRNA水平会因葡萄糖利用的改变而以相同方式变化；2）负责SI基因葡萄糖依赖性转录的调控元件位于启动子的-370/+30区域内。
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