在表达水稻同源异型盒基因OSH1的烟草植株中，对表达增强的cDNA克隆进行分离与鉴定。
Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene, OSH1.
作者信息
Tamaoki M, Yamamoto N, Matsuoka M
机构信息
Nagoya University, BioScience Center, Aichi, Japan.
出版信息
Plant Cell Physiol. 1997 May;38(5):638-42. doi: 10.1093/oxfordjournals.pcp.a029215.
Abstract
We have used subtractive hybridization to isolate cDNA clones whose expression were up-regulated in transgenic tobacco ectopically expressing the rice homeobox gene, OSH1. Thirty-nine distinct cDNA clones, which we term HRGs (Homeobox Regulated Genes), were identified. Some of them were specifically expressed in transformants, indicating that their expression was possibly regulated by transgene.
摘要
我们利用消减杂交技术分离出了一些cDNA克隆，这些克隆在异位表达水稻同源异型框基因OSH1的转基因烟草中表达上调。我们鉴定出了39个不同的cDNA克隆，将其命名为HRGs（同源异型框调控基因）。其中一些在转化体中特异性表达，表明它们的表达可能受转基因调控。
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