Schmiedel D, Vary P S, Jablonski L, Hillen W
Lehrstuhl für Mikrobiologie, Universität Erlangen-Nürnberg, Germany.
Appl Microbiol Biotechnol. 1997 May;47(5):543-6. doi: 10.1007/s002530050970.
We constructed integrative plasmids to place xylA-lacZ indicator gene fusions into two different loci of the Bacillus megaterium chromosome, gdh2 and trpC, in lac mutants of strains DSM 319 and QM B1551, which differ markedly. Single-crossover integration was achieved in all cases while double crossovers occurred only in gdh2 of DSM 319 and QM B1551 and in trpC of QM B1551. Neither of the loci affected regulation of the xylA-lacZ fusions. These results confirm the suitability of the two gene loci for single-copy cloning.
我们构建了整合质粒，将木糖A-乳糖Z指示基因融合体置于巨大芽孢杆菌染色体的两个不同位点，即gdh2和trpC，在菌株DSM 319和QM B1551的乳糖突变体中，这两个菌株有显著差异。在所有情况下都实现了单交换整合，而双交换仅发生在DSM 319和QM B1551的gdh2以及QM B1551的trpC中。这两个位点均未影响木糖A-乳糖Z融合体的调控。这些结果证实了这两个基因位点适用于单拷贝克隆。
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