Expanded bed adsorption for recovery of renatured human recombinant interleukin 8 from Escherichia coli inclusion bodies.

Expanded bed adsorption is a chromatography technique based on stable fluidization, designed for large scale recovery of proteins directly from particulate-containing feedstocks. Described in this article is the use of expanded bed adsorption for recovery of renatured recombinant human interleukin 8 from Escherichia coli inclusion bodies, using a cation exchange adsorbent. The yield of interleukin 8 from the expanded bed was approximately 100% and the purification factor was approximately 4. Following each interleukin 8 purification a cleaning in place procedure was performed with a commercial cleaner. The results show that the adsorbent can be used at least 50 times without any significant loss of function.