Barnfield Frej A K
Pharmacia Biotech AB, Uppsala, Sweden.
Bioseparation. 1996;6(5):265-71.
Expanded bed adsorption is a chromatography technique based on stable fluidization, designed for large scale recovery of proteins directly from particulate-containing feedstocks. Described in this article is the use of expanded bed adsorption for recovery of renatured recombinant human interleukin 8 from Escherichia coli inclusion bodies, using a cation exchange adsorbent. The yield of interleukin 8 from the expanded bed was approximately 100% and the purification factor was approximately 4. Following each interleukin 8 purification a cleaning in place procedure was performed with a commercial cleaner. The results show that the adsorbent can be used at least 50 times without any significant loss of function.
扩张床吸附是一种基于稳定流化的色谱技术，旨在直接从含颗粒的原料中大规模回收蛋白质。本文描述了使用阳离子交换吸附剂通过扩张床吸附从大肠杆菌包涵体中回收复性重组人白细胞介素8的方法。从扩张床中回收的白细胞介素8的产率约为100%，纯化因子约为4。每次白细胞介素8纯化后，使用市售清洁剂进行在位清洗程序。结果表明，该吸附剂可使用至少50次而功能无任何显著损失。
