Rigacci S, Iantomasi T, Marraccini P, Berti A, Vincenzini M T, Ramponi G
Department of Biochemical Sciences, University of Firenze, Viale Morgagni 50, 50134 Firenze, Italy.
Biochem J. 1997 Jun 15;324 ( Pt 3)(Pt 3):791-6. doi: 10.1042/bj3240791.
Recent studies show that glutathione, while being involved in the well-known physiological processes of amino acid transport and detoxification, can also play a part in cell proliferation events. Cell treatment with l-buthionine sulphoximine, which causes glutathione depletion, is accompanied by a decrease in cell proliferation. At present no precise relationship between this thiol and any critical intermediate of the mitogenic cascade has been proved. In this study, conducted on NIH/3T3 murine fibroblasts, we demonstrate a strict correlation between glutathione levels and platelet-derived growth-factor-receptor activation in response to stimulation and cell proliferation. The receptor autophosphorylation is severely impaired at low glutathione cellular levels. The interaction of glutathione with this growth-factor receptor in vivo, while being rather specific, is complex and may involve both cytosolic and extracellular receptor domains.
最近的研究表明，谷胱甘肽虽然参与了众所周知的氨基酸转运和解毒生理过程，但也能在细胞增殖活动中发挥作用。用L-丁硫氨酸亚砜胺处理细胞会导致谷胱甘肽耗竭，同时伴随着细胞增殖的减少。目前尚未证实这种硫醇与有丝分裂原级联反应的任何关键中间体之间存在精确关系。在这项对NIH/3T3小鼠成纤维细胞进行的研究中，我们证明了谷胱甘肽水平与血小板衍生生长因子受体在刺激反应和细胞增殖中的激活之间存在严格的相关性。在低谷胱甘肽细胞水平下，受体自磷酸化严重受损。谷胱甘肽与这种生长因子受体在体内的相互作用虽然相当特异，但很复杂，可能涉及胞质和细胞外受体结构域。
