Armstrong R C, Migneault A, Shegog M L, Kim J G, Hudson L D, Hessler R B
Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799, USA.
J Neuropathol Exp Neurol. 1997 Jul;56(7):772-81.
Detection and characterization of distinct central nervous system (CNS) tumor cell types is clinically important since distinct tumor types are associated with different prognoses and treatments. However, there is currently a lack of markers to identify certain glioma types and insufficient understanding as to which cells give rise to different glioma cell types. In the present study, biopsy specimens from human brain tumors were analyzed for expression of Myelin Transcription Factor 1 (MYT1) to explore the extent to which glioma cells reflect characteristic expression of MYT1 in developing glial progenitor cells. Immunostaining with an antibody against MYT1 revealed widespread immunoreactivity that was most prominent in high-grade oligodendrogliomas, astrocytomas, and mixed oligoastrocytomas as well as in a dysembryoplastic neuroepithelial tumor. MYT1 immunoreactivity in tumor regions generally correlated with the prevalence of cells exhibiting nuclear immunolabeling with an antibody against Ki-67, suggesting an association of MYT1 with cell proliferation that was also observed in normal adult human and rat brain in the germinal subependymal zone. The MYT1 immunoreactivity was frequently nuclear, appearing as dotted or punctate, but in some cases it was localized to the cytoplasm. In combination with histopathological studies and analysis of Ki-67 immunoreactivity, examination of MYT1 immunolabeling may provide additional information to aid in the detection and diagnosis of CNS tumors.
鉴别和表征不同的中枢神经系统（CNS）肿瘤细胞类型具有重要的临床意义，因为不同的肿瘤类型与不同的预后和治疗方法相关。然而，目前缺乏用于识别某些胶质瘤类型的标志物，并且对于哪些细胞产生不同的胶质瘤细胞类型了解不足。在本研究中，分析了人脑肿瘤活检标本中髓磷脂转录因子1（MYT1）的表达，以探讨胶质瘤细胞在多大程度上反映了发育中的神经胶质祖细胞中MYT1的特征性表达。用抗MYT1抗体进行免疫染色显示广泛的免疫反应性，在高级别少突胶质细胞瘤、星形细胞瘤、混合性少突星形细胞瘤以及胚胎发育不良性神经上皮肿瘤中最为明显。肿瘤区域的MYT1免疫反应性通常与用抗Ki-67抗体显示核免疫标记的细胞的患病率相关，这表明MYT1与细胞增殖有关，在正常成人和大鼠脑的生发室管膜下区也观察到这种关联。MYT1免疫反应性通常位于细胞核，呈点状或斑状，但在某些情况下定位于细胞质。结合组织病理学研究和Ki-67免疫反应性分析，检测MYT1免疫标记可能为中枢神经系统肿瘤的检测和诊断提供额外信息。
