用于检测瘟病毒的cDNA探针。

cDNA probes for the detection of pestiviruses.

作者信息

Cruciere C, Bakkali L, Gonzague M, Plateau E

机构信息

Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire Central de Recherches Vétérinaires, (C.N.E.V.A./L.C.R.V.), France.

出版信息

Arch Virol Suppl. 1991;3:191-7. doi: 10.1007/978-3-7091-9153-8_23.

DOI:10.1007/978-3-7091-9153-8_23

PMID:9210941

Abstract

Probes were prepared from genomic RNA of Hog Cholera Virus (HCV) after synthesis of cDNA and cloning. Six probes were selected according to their place on the viral genome determined by sequencing and comparison with BVDV sequence. These probes were hybridized with two strains of HCV (Alfort and Nord), two strains of Bovine Viral Diarrhea (BVDV) (NADL, New York) and four strains of Border Disease (BD) (Lyon 1, Lyon 2, Aveyron, IEMVT). This panel of six probes seem to be able to differentiate pestiviruses but some differences rely only on slight intensity of the hybridization.

摘要

在合成 cDNA 并克隆后，从猪瘟病毒（HCV）的基因组 RNA 制备探针。根据测序确定的探针在病毒基因组上的位置并与牛病毒性腹泻病毒（BVDV）序列进行比较，选择了六个探针。这些探针与两株 HCV（阿尔福特株和诺德株）、两株牛病毒性腹泻病毒（BVDV）（NADL 株、纽约株）以及四株边境病病毒（BD）（里昂 1 株、里昂 2 株、阿韦龙株、IEMVT 株）进行杂交。这一组六个探针似乎能够区分瘟病毒，但有些差异仅体现在杂交强度略有不同。