Hiscott P, Seitz B, Schlötzer-Schrehardt U, Naumann G O
Unit of Ophthalmology, Department of Medicine and Department of Pathology, University of Liverpool, Liverpool, UK.
Cell Tissue Res. 1997 Aug;289(2):307-10. doi: 10.1007/s004410050877.
Light- and electron-microscopic immunohistochemical techniques were used to investigate the distribution of the matricellular protein thrombospondin 1 in normal human, bovine and rabbit cornea. Light-microscopic immunoreactivity for thrombospondin 1 was observed in the epithelial basement membrane, posterior Descemet's membrane and endothelium of human and bovine cornea. The bulk of the stroma, the stromal cells (keratocytes) and the anterior part of Descemet's membrane in human and bovine cornea were devoid of detectable thrombospondin 1 and the protein could not be demonstrated in any of the layers of the rabbit cornea. Electron-microscopic immunogold studies of human and bovine cornea revealed that thrombospondin 1 labelling of corneal endothelial (and basal epithelial) cells included focal deposits at cell membranes. It is postulated that thrombospondin 1 regulates interactions between cells and their basement membrane, and perhaps cell-to-cell interactions, in the normal human and bovine corneal endothelium and basal epithelium.
采用光镜和电镜免疫组织化学技术研究了基质细胞蛋白血小板反应蛋白1在正常人、牛和兔角膜中的分布。在人及牛角膜的上皮基底膜、后弹力层和内皮中观察到血小板反应蛋白1的光镜免疫反应性。人及牛角膜的大部分基质、基质细胞（角膜细胞）和前弹力层均未检测到血小板反应蛋白1，且在兔角膜的任何层中均未证实有该蛋白。对人及牛角膜的电镜免疫金研究显示，角膜内皮（和基底上皮）细胞的血小板反应蛋白1标记包括细胞膜上的局灶性沉积。推测血小板反应蛋白1在正常人及牛角膜内皮和基底上皮中调节细胞与其基底膜之间的相互作用，可能还调节细胞间相互作用。
