Frankel S S, Tenner-Racz K, Racz P, Wenig B M, Hansen C H, Heffner D, Nelson A M, Pope M, Steinman R M
Armed Forces Institute of Pathology, Walter Reed Army Institute of Research, Rockville, Maryland, USA.
Am J Pathol. 1997 Jul;151(1):89-96.
Cells that are infected with HIV-1 were visualized at the mucosal surface of the nasopharyngeal and palatine tonsils in 14 specimens from patients with CD4+ T-cell counts of 200 to 900/microliter and 2- to 10-year histories of HIV-1 infection. Most of the cells with intracellular HIV-1 protein were small but multinucleated. The majority of these syncytia could be double labeled for HIV-1 RNA and a dendritic cell marker S100. In the palatine tonsil, the infected cells were not found in the stratified squamous epithelium that is adjacent to the pharynx. Instead, the S100+ infected syncytia were localized to the surface of tonsil invaginations or crypts. This mucosa, termed lymphoepithelium, contains antigen-transporting M cells that lie above regions where S100+ dendritic cells are juxtaposed with CD4+ lymphocytes. Likewise, infected cells were found in lymphoepithelium and not respiratory epithelium of nasopharyngeal tonsils or adenoids. We propose that lymphoepithelia, the histological term that describes the specialized regions where antigens access mucosa-associated lymphoid tissue, are sites where HIV-1 replication can be enhanced in syncytia derived from dendritic cells.
在14例CD4 + T细胞计数为200至900/微升且有2至10年HIV-1感染史的患者标本中，观察到了感染HIV-1的细胞位于鼻咽和腭扁桃体的粘膜表面。大多数含有细胞内HIV-1蛋白的细胞较小但为多核。这些多核巨细胞中的大多数可以用HIV-1 RNA和树突状细胞标志物S100进行双重标记。在腭扁桃体中，未在与咽部相邻的复层鳞状上皮中发现感染细胞。相反，S100 +感染的多核巨细胞定位于扁桃体隐窝或隐窝的表面。这种被称为淋巴上皮的粘膜含有抗原转运M细胞，这些细胞位于S100 +树突状细胞与CD4 +淋巴细胞并列的区域上方。同样，在淋巴上皮中发现了感染细胞，而在鼻咽扁桃体或腺样体的呼吸道上皮中未发现。我们提出，淋巴上皮是描述抗原进入粘膜相关淋巴组织的特殊区域的组织学术语，是HIV-1在源自树突状细胞的多核巨细胞中复制增强的部位。
