Shimada K, Kamezawa Y, Mitamura K
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Biol Pharm Bull. 1997 Jun;20(6):596-600. doi: 10.1248/bpb.20.596.
In vitro glucuronidation of 25-hydroxyvitamin D3 and its pro-form has been investigated by means of HPLC with UV detection. Although both substrates gave 3- and 25-glucuronides in the presence of the rat liver microsomal fraction and uridine-5'-diphosphoglucuronic acid, 25-hydroxyvitamin D3 and its pro-form yielded 3- and 25-glucuronide as the main product, respectively. The latter glucuronide is the one in which the tert-hydroxy group is conjugated. Each glucuromide was identified by its chromatographic behavior in comparison with an authentic sample and data obtained from liquid chromatography/mass spectrometry (LC/MS) using atmospheric pressure chemical ionization.
通过带有紫外检测的高效液相色谱法研究了25-羟基维生素D3及其前体形式的体外葡萄糖醛酸化作用。尽管在大鼠肝脏微粒体组分和尿苷-5'-二磷酸葡萄糖醛酸存在的情况下，两种底物都生成了3-和25-葡萄糖醛酸苷，但25-羟基维生素D3及其前体形式分别以3-和25-葡萄糖醛酸苷作为主要产物。后一种葡萄糖醛酸苷是叔羟基被结合的那种。通过与标准样品的色谱行为以及使用大气压化学电离的液相色谱/质谱（LC/MS）获得的数据进行比较，鉴定了每种葡萄糖醛酸苷。
