铜绿假单胞菌中的钠（锂）/氢反向转运体及锂对细胞生长的影响

Na+(Li+)/H+ antiporter in Pseudomonas aeruginosa and effect of Li+ on cell growth.

作者信息

Inaba K, Utsugi J, Kuroda T, Tsuda M, Tsuchiya T

机构信息

Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.

出版信息

Biol Pharm Bull. 1997 Jun;20(6):621-4. doi: 10.1248/bpb.20.621.

DOI:10.1248/bpb.20.621

PMID:9212978

Abstract

Everted membrane vesicles were prepared from cells of Pseudomonas aeruginosa, and cation/H+ antiport was measured. We observed activities of Na+/H+ antiport, Li+/H+ antiport and K+/H+ antiport. Judging from the competition pattern, it seems that there are at least two types of antiporter, a Na+(Li+)/H+ antiporter and a K+/H+ antiporter. Na+ was a good substrate for the Na+(Li+)/H+ system, whereas Li+ was a poor substrate. Although the K(m) value for Na+ (or Li+) was similar to those in Escherichia coli Na+/H+ antiporters, the Vmax value for Na+ (or Li+) was much smaller in the P. aeruginosa antiporter than in the E. coli antiporters. Growth of P. aeruginosa was strongly inhibited by 0.4 M LiCl, but not by NaCl or KCl.

摘要

从铜绿假单胞菌细胞制备外翻膜泡，并测定阳离子/H⁺反向转运。我们观察到了Na⁺/H⁺反向转运、Li⁺/H⁺反向转运和K⁺/H⁺反向转运的活性。从竞争模式判断，似乎至少有两种类型的反向转运体，一种是Na⁺(Li⁺)/H⁺反向转运体和一种K⁺/H⁺反向转运体。Na⁺是Na⁺(Li⁺)/H⁺系统的良好底物，而Li⁺是较差的底物。尽管Na⁺(或Li⁺)的K(m)值与大肠杆菌Na⁺/H⁺反向转运体中的相似，但铜绿假单胞菌反向转运体中Na⁺(或Li⁺) 的Vmax值比大肠杆菌反向转运体中的小得多。0.4 M LiCl强烈抑制铜绿假单胞菌的生长，但NaCl或KCl则无此作用。