Hayashi C, Fujino H, Ogata M, Sato Y, Iguchi-Ariga S M, Ariga H
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Biol Pharm Bull. 1997 Jun;20(6):690-3. doi: 10.1248/bpb.20.690.
We previously isolated from mouse cells an autonomous replicating sequence (ARS) ARS65 (Ariga, Itani and Iguchi-Ariga, Mol. Cell. Biol. 7, 1-6, 1987). Here we report the nucleotide sequence of ARS65. The sequence from BgIII to EcoRI sites cloned as ARS was 2658 bp long. There exist three interesting domains: a TA repeat, a myc like box (essential sequence for c-myc ARS), and a T rich region. Cloned DNAs containing various segments of pARS65 were transfected to rat 3Y1 cells together with the hygromycinB resistance expression vector, and hygromycinB resistant clones were isolated. Established cell lines transfected with plasmids carrying either a myc-like box or a T rich region harbored the replicated plasmids, indicating that these two elements are necessary for the ARS function of pARS65.
我们之前从小鼠细胞中分离出一个自主复制序列（ARS）ARS65（有贺、伊谷和井口有贺，《分子细胞生物学》7卷，第1 - 6页，1987年）。在此我们报告ARS65的核苷酸序列。作为ARS克隆的从BgIII到EcoRI位点的序列长2658 bp。存在三个有趣的结构域：一个TA重复序列、一个类myc盒（c - myc ARS的必需序列）和一个富含T的区域。将含有pARS65不同片段的克隆DNA与潮霉素B抗性表达载体一起转染到大鼠3Y1细胞中，并分离出潮霉素B抗性克隆。用携带类myc盒或富含T区域的质粒转染建立的细胞系含有复制的质粒，表明这两个元件对于pARS65的ARS功能是必需的。
