Lankat-Buttgereit B, Göke B
Department of Internal Medicine, Philipps-University of Marburg, Germany.
Peptides. 1997;18(5):617-24. doi: 10.1016/s0196-9781(97)00001-6.
The glucagon-like peptide 1 (7-36) amide (GLP-1) receptor mediates the insulinotropic effects of the incretin hormone GLP-1. To elucidate the tissue-specific regulation of the GLP-1 receptor we screened a human genomic library with a human GLP-1 receptor cDNA. The gene spans 40 kb and consists of at least seven exons. The promoter contained no TATA- or CAAT-boxes, but several other putative cis-regulatory recognition sequences including three Sp1 binding sites. Transient transfections of GLP-1 receptor producing and non-producing cells with promoter/ reporter gene constructs revealed that the putative Sp1 binding sites and several other silencer and tissue specific elements are important for the activity. Therefore, 3000 bp upstream the GLP-1 receptor coding sequences comprise regulatory elements essential for the tissue- and cell-specific transcription of the gene.
胰高血糖素样肽1（7 - 36）酰胺（GLP - 1）受体介导肠促胰岛素激素GLP - 1的促胰岛素作用。为阐明GLP - 1受体的组织特异性调控，我们用人GLP - 1受体cDNA筛选了一个人类基因组文库。该基因跨度为40 kb，至少由七个外显子组成。其启动子不含TATA盒或CAAT盒，但含有其他几个推定的顺式调控识别序列，包括三个Sp1结合位点。用启动子/报告基因构建体对产生和不产生GLP - 1受体的细胞进行瞬时转染，结果表明推定的Sp1结合位点以及其他几个沉默子和组织特异性元件对活性很重要。因此，GLP - 1受体编码序列上游3000 bp包含该基因组织和细胞特异性转录所必需的调控元件。
