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Structural analysis of disaccharides synthesized by beta-D-glucosidase of Bifidobacterium breve clb and their assimilation by Bifidobacteria.

作者信息

Nunoura N, Fujita T, Ohdan K, Kirihata M, Yamamoto K, Kumagai H

机构信息

Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.

出版信息

Biosci Biotechnol Biochem. 1997 Jun;61(6):1033-5. doi: 10.1271/bbb.61.1033.

DOI:10.1271/bbb.61.1033
PMID:9214768
Abstract

Circulation of a solution of 1 M D-fucose and 1 M D-glucose through a reaction system consisting of serial columns of immobilized recombinant beta-D-glucosidase of Bifidobacterium breve clb and activated charcoal gave two oligosaccharides. Structural analysis identified these oligosaccharides as D-fucosylglucose (6-O-beta-D-Fucopyranosyl-D-glucose) and gentiobiose (6-O-beta-D-Glucopyranosyl-D-glucose). The D-fucosylglucose obtained was well assimilated by many Bifidobacteria but not by the other intestinal bacteria tested.

摘要

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