Chao Q, Rothenberg M, Solano R, Roman G, Terzaghi W, Ecker J R
Department of Biology, Plant Science Institute, University of Pennsylvania, Philadelphia 19104-6018, USA.
Cell. 1997 Jun 27;89(7):1133-44. doi: 10.1016/s0092-8674(00)80300-1.
Mutations in the Arabidopsis ETHYLENE-INSENSITIVE3 (EIN3) gene severely limit a plant's response to the gaseous hormone ethylene. ein3 mutants show a loss of ethylene-mediated effects including gene expression, the triple response, cell growth inhibition, and accelerated senescence. EIN3 acts downstream of the histidine kinase ethylene receptor, ETR1, and the Raf-like kinase, CTR1. The EIN3 gene encodes a novel nuclear-localized protein that shares sequence similarity, structural features, and genetic function with three EIN3-LIKE (EIL) proteins. In addition to EIN3, EIL1 orEIL2 were able to complement ein3, suggesting their participation in the ethylene signaling pathway. Overexpression of EIN3 or EIL1 in wild-type or ethylene-insensitive2 plants conferred constitutive ethylene phenotypes, indicating their sufficiency for activation of the pathway in the absence of ethylene.
拟南芥乙烯不敏感3（EIN3）基因的突变严重限制了植物对气态激素乙烯的反应。ein3突变体表现出乙烯介导的效应丧失，包括基因表达、三重反应、细胞生长抑制和加速衰老。EIN3作用于组氨酸激酶乙烯受体ETR1和类Raf激酶CTR1的下游。EIN3基因编码一种新的核定位蛋白，该蛋白与三种EIN3样（EIL）蛋白具有序列相似性、结构特征和遗传功能。除EIN3外，EIL1或EIL2能够互补ein3，表明它们参与乙烯信号通路。在野生型或乙烯不敏感2型植物中过表达EIN3或EIL1会赋予组成型乙烯表型，表明它们在没有乙烯的情况下足以激活该通路。
