四环素毛细管电泳的优化方法。

Optimized methods for capillary electrophoresis of tetracyclines.

作者信息

Li Y M, Van Schepdael A, Roets E, Hoogmartens J

机构信息

Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, K.U. Leuven, Belgium.

出版信息

J Pharm Biomed Anal. 1997 May;15(8):1063-9. doi: 10.1016/s0731-7085(96)01995-4.

DOI:10.1016/s0731-7085(96)01995-4

PMID:9215958

Abstract

Optimized methods for the analysis of some tetracyclines by capillary electrophoresis are described. Different buffer systems were employed for the separation of tetracycline, oxytetracycline and demeclocycline from their respective major impurities, including the 2-acetyl-2-decarboxamido derivatives. The influence of buffer pH and buffer concentration was systematically investigated. Non-ionic surfactant Triton X-100 and methyl-beta-cyclodextrin were used to obtain improved selectivity in the case of oxytetracycline and demeclocycline. The results are compared with those of previously established liquid chromatography methods. Good correlations were obtained.

摘要

描述了用于毛细管电泳分析某些四环素的优化方法。采用不同的缓冲体系从各自的主要杂质（包括2-乙酰基-2-脱羧酰胺衍生物）中分离四环素、土霉素和地美环素。系统研究了缓冲液pH值和缓冲液浓度的影响。在土霉素和地美环素的情况下，使用非离子表面活性剂Triton X-100和甲基-β-环糊精来提高选择性。将结果与先前建立的液相色谱方法的结果进行了比较。获得了良好的相关性。