Tsou T L, Wu J R, Young C D, Wang T M
Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.
J Pharm Biomed Anal. 1997 May;15(8):1197-205. doi: 10.1016/s0731-7085(96)01960-7.
A simple, rapid and accurate method for simultaneous determination of amoxycillin and clavulanic acid using HPLC with beta-cyclodextrin stationary phase was developed. It involves the use of tetraethylammonium acetate (TEAA) as an additive reagent, methanol-buffer solution (pH 4.5) (35:65; v/v) as the mobile phase, detection at 225 mm and chromatogram within 12 min. Linearity and precision of the internal standard method have been obtained. Recoveries ranged from 99.25 to 105.63% for amoxycillin in the synthetic mixture. For clavulanic acid it was from 99.50 to 101.64%. This method is convenient and reproducible for analyses of these two components in different dosage forms.
