Ruck A, Paulie S
Department of Immunology, Stockholm University, Sweden.
Anticancer Res. 1997 May-Jun;17(3C):1925-31.
We have previously shown that human bladder tumor cell lines may be adapted to grow in the complete absence of serum or any other growth supplement and that this can be explained on the basis of autocrine stimulation. In the present study we have extended the number of cell lines that could be established as serum-free cultures and found this capacity to be correlated with tumor malignancy. We also used the receptor blocking monoclonal antibody, mAb 528, to study its effect on tumor cell growth. Inhibition was observed in all of seven bladder carcinoma cell lines tested. A similar effect was observed in two colon carcinoma cell lines but not in a melanoma line. The results show that the EGFR is involved in autocrine growth stimulation and that the acquirement of autonomous growth capacity is likely to be an important factor in the oncogenesis of bladder tumors.
我们之前已经表明，人类膀胱肿瘤细胞系可以在完全没有血清或任何其他生长补充剂的情况下适应生长，并且这可以基于自分泌刺激来解释。在本研究中，我们扩展了可以建立无血清培养的细胞系数量，并发现这种能力与肿瘤恶性程度相关。我们还使用了受体阻断单克隆抗体mAb 528来研究其对肿瘤细胞生长的影响。在所测试的七个膀胱癌细胞系中均观察到抑制作用。在两个结肠癌细胞系中观察到了类似的效果，但在一个黑色素瘤细胞系中未观察到。结果表明，表皮生长因子受体（EGFR）参与自分泌生长刺激，并且获得自主生长能力可能是膀胱肿瘤发生的一个重要因素。
