Cubillos S, Scallon B, Feldmann M, Taylor P
Laboratorio de Fisiopatologia, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas, Venezuela.
Anticancer Res. 1997 May-Jun;17(3C):2207-11.
We studied the relationship between tumour necrosis factor (TNF) and interleukin 6 (IL-6) levels, and the metastatic process in C57BL/6 mice after intravenous inoculation of B16-BL6 melanoma cells. Bioactive TNF was not detectable in the sera of inoculated mice, but these animals did show higher TNF levels following intraperitoneal challenge with lipopolysaccharide (LPS) compared to control animals. Serum IL-6 levels were increased in inoculated animals. Injection of a hybrid molecule (p55-sf2) composed of the human p55 TNF receptor extracellular domain coupled to a human constant region backbone, decreased serum TNF (after LPS challenge) and IL-6 levels in inoculated animals. Lung metastases at 7-14 days were reduced, compared to human IgG-injected control animals, but this effect was lost at day 21 postinoculation. The results suggest that the reduction in the number of metastases may be related to the effect of blocking TNF activity.
我们研究了静脉接种B16-BL6黑色素瘤细胞后，C57BL/6小鼠体内肿瘤坏死因子（TNF）和白细胞介素6（IL-6）水平与转移过程之间的关系。在接种小鼠的血清中未检测到生物活性TNF，但与对照动物相比，这些动物在腹腔注射脂多糖（LPS）后确实显示出更高的TNF水平。接种动物的血清IL-6水平升高。注射由人p55 TNF受体胞外域与人恒定区骨架偶联组成的杂合分子（p55-sf2），可降低接种动物血清TNF（LPS攻击后）和IL-6水平。与注射人IgG的对照动物相比，接种后7-14天肺转移减少，但在接种后第21天这种效果消失。结果表明，转移灶数量的减少可能与阻断TNF活性的作用有关。
