Figueiredo D M, Hallewell R A, Chen L L, Fairweather N F, Dougan G, Savitt J M, Parks D A, Fishman P S
Department of Biochemistry, Imperial College, London, United Kingdom.
Exp Neurol. 1997 Jun;145(2 Pt 1):546-54. doi: 10.1006/exnr.1997.6490.
The nontoxic C fragment of tetanus toxin (TC) can transport other proteins from the circulation to central nervous system (CNS) motor neurons. Increased levels of CuZn superoxide dismutase (SOD) are protective in experimental models of stroke and Parkinson's disease, whereas mutations in SOD can cause motor neuron disease. We have linked TC to SOD and purified the active recombinant proteins in both the TC-SOD and SOD-TC orientations. Light microscopic immunohistochemistry and quantitative enzyme-linked immunosorbant assays (ELISA) of mouse brainstem, after intramuscular injection, demonstrate that the fusion proteins undergo retrograde axonal transport and transsynaptic transfer as efficiently as TC alone.
破伤风毒素（TC）的无毒C片段可将其他蛋白质从循环系统转运至中枢神经系统（CNS）的运动神经元。铜锌超氧化物歧化酶（SOD）水平升高在中风和帕金森病的实验模型中具有保护作用，而SOD突变可导致运动神经元疾病。我们已将TC与SOD连接，并以TC-SOD和SOD-TC两种方向纯化了活性重组蛋白。肌肉注射后，对小鼠脑干进行光学显微镜免疫组织化学和定量酶联免疫吸附测定（ELISA），结果表明融合蛋白与单独的TC一样有效地进行逆行轴突运输和跨突触转移。
