犬实验性蛛网膜下腔出血后脑实质内小动脉的形态学变化

Morphological changes of intraparenchymal arterioles after experimental subarachnoid hemorrhage in dogs.

作者信息

Ohkuma H, Itoh K, Shibata S, Suzuki S

机构信息

Department of Neurosurgery, Hirosaki University School of Medicine, Japan.

出版信息

Neurosurgery. 1997 Jul;41(1):230-5; discussion 235-6. doi: 10.1097/00006123-199707000-00036.

DOI:10.1097/00006123-199707000-00036

PMID:9218311

Abstract

OBJECTIVE

Morphological and microcirculatory changes in intraparenchymal vessels after subarachnoid hemorrhage (SAH) have not yet been fully clarified. We conducted this experimental study to investigate the serial morphological changes of intraparenchymal arterioles after SAH.

METHODS

SAH was produced by injecting autologous arterial blood into the cisterna magna twice at 48-hour intervals in 30 dogs. The dogs were killed 3, 7, or 14 days after SAH, and then perfusion-fixed specimens of both anterior sylvian giri were obtained by using two methods. Microvascular corrosion casts produced by arterial injection of polyester resin were examined using scanning electron microscopy, and the widths of 40 arterioles of each animal were measured. Sectioned slices from the brain surface to 500 microns deep were examined by light microscopy, and external diameter, internal diameters, and wall thickness of the arterioles at depths of 50, 200, and 500 microns from the brain surface were morphometrically evaluated in 40 arterioles of each animal. In control animals receiving cisternal injections of mock cerebrospinal fluid (n = 10) and in healthy control animals (n = 10), the same examination and evaluation were performed.

RESULTS

Corrosion casts of arterioles showed tapered narrowing with folding after SAH, and the width of the arterioles significantly decreased 3 and 7 days after SAH (P < 0.01). Morphometric examination by light microscopy showed a significant decrease of internal diameter of arterioles associated with a significant increase of wall thickness at any depth from the brain surface 3 and 7 days after SAH (P < 0.05 or P < 0.01). These findings improved 14 days after SAH. Control animals receiving cisternal injections of mock cerebrospinal fluid showed no significant differences compared with healthy control animals.

CONCLUSION

These results suggest that constriction of intraparenchymal arterioles occurs after SAH and may contribute to delayed cerebral ischemia.

摘要

目的

蛛网膜下腔出血（SAH）后脑实质内血管的形态学和微循环变化尚未完全阐明。我们进行了这项实验研究，以探讨SAH后脑实质内小动脉的系列形态学变化。

方法

对30只犬每隔48小时向小脑延髓池注射自体动脉血两次，制造SAH模型。在SAH后3、7或14天处死犬，然后用两种方法获取双侧大脑外侧裂的灌注固定标本。通过动脉注射聚酯树脂制作微血管铸型，用扫描电子显微镜检查，并测量每只动物40条小动脉的宽度。从脑表面至500微米深处的切片用光学显微镜检查，对每只动物40条小动脉在距脑表面50、200和500微米深处的外径、内径和壁厚进行形态学评估。对接受小脑延髓池注射模拟脑脊液的对照动物（n = 10）和健康对照动物（n = 10）进行相同的检查和评估。

结果

SAH后小动脉铸型显示逐渐变细并伴有折叠，SAH后3天和7天小动脉宽度显著减小（P < 0.01）。光学显微镜下的形态学检查显示，SAH后3天和7天，在距脑表面任何深度处，小动脉内径显著减小，壁厚显著增加（P < 0.05或P < 0.01）。这些变化在SAH后14天有所改善。接受小脑延髓池注射模拟脑脊液的对照动物与健康对照动物相比无显著差异。