Krieger N R, Ito H, Fathman C G
Department of Surgery, Stanford University School of Medicine, CA, USA.
J Autoimmun. 1997 Jun;10(3):309-15. doi: 10.1006/jaut.1997.0126.
The relative contributions of the CD4+ and CD8+ T cell subpopulations in xenotransplant rejection were studied using CD4 and CD8 knockout (KO) mice. Wistar Furth (WF, RT1a) rat pancreatic islet or skin xenografts were transplanted into either CD4 or CD8 KO recipients and compared to wild-type controls. Long-term survival of WF islet xenografts was observed in the CD4 KO mice (MST, >66+/-8 days) whereas CD8 KO mice rejected their islet xenografts within 8 days, similar to controls (MST, 7+/-0.2 days). In contrast, WF skin xenografts were rejected in both CD4 and CD8 KO recipients within 8 days. CD4 KO recipients which maintained xenoislets >90 days posttransplant rejected WF skin grafts within 9 days, without rejecting their original islet xenografts. These results suggest that CD4+ cells are essential for mediating islet xenograft rejection. These data also suggest that the absence of either CD4+ or CD8+ T cells is not sufficient to prevent rejection of skin xenografts.
利用CD4和CD8基因敲除（KO）小鼠研究了CD4⁺和CD8⁺T细胞亚群在异种移植排斥反应中的相对作用。将Wistar Furth（WF，RT1a）大鼠胰岛或皮肤异种移植物移植到CD4或CD8基因敲除受体中，并与野生型对照进行比较。在CD4基因敲除小鼠中观察到WF胰岛异种移植物的长期存活（中位生存时间，>66±8天），而CD8基因敲除小鼠在8天内排斥其胰岛异种移植物，与对照相似（中位生存时间，7±0.2天）。相反，WF皮肤异种移植物在CD4和CD8基因敲除受体中均在8天内被排斥。移植后维持异种胰岛>90天的CD4基因敲除受体在9天内排斥WF皮肤移植物，而未排斥其原有的胰岛异种移植物。这些结果表明，CD4⁺细胞对于介导胰岛异种移植排斥至关重要。这些数据还表明，缺乏CD4⁺或CD8⁺T细胞不足以防止皮肤异种移植物的排斥。
