Cadenhead D A, Kellner B M, Jacobson K, Papahadjopoulos D
Biochemistry. 1977 Nov 29;16(24):5386-92. doi: 10.1021/bi00643a034.
A study of three fluorescent anthroyl probes has been carried out using pure and mixed monomolecular films with dipalmitoylphosphatidylcholine. In addition, fluorescence depolarization and differential scanning calorimetry data were obtained from dipalmitoylphosphatidylcholine vesicles with incorporated anthroyl probes. The three probes used were 2-(9-anthroyl)palmitic acid. 12-(9-anthroyl)stearic acid, and 16-(9-anthroyl)palmitic acid. The latter probe was synthesized for these studies. In monolayers the probes shifted the onset of the liquid-condensed/liquid-expanded monolayer phase transition with the extent of the shift decreasing in the order: 2-(9-anthroyl)palmitic acid greater than 12-(9-anthroyl)stearic acid greater than 16-(9-anthroyl)stearic acid. A corresponding decrease in the gel-liquid crystalline bilayer transition temperature (Tc) showed the same order of perturbation in both the fluorescence depolarization and differential scanning calorimetry data. Locating the anthroyl entity in the center of the bilayer would appear to provide a minimum perturbation.
利用二棕榈酰磷脂酰胆碱的纯单分子膜和混合单分子膜对三种荧光蒽基探针进行了研究。此外，还从掺入蒽基探针的二棕榈酰磷脂酰胆碱囊泡中获得了荧光去极化和差示扫描量热数据。所使用的三种探针分别是2-(9-蒽基)棕榈酸、12-(9-蒽基)硬脂酸和16-(9-蒽基)棕榈酸。后一种探针是为这些研究而合成的。在单分子层中，探针使液晶态/液态扩展单分子层相变的起始点发生了移动，移动程度按以下顺序降低：2-(9-蒽基)棕榈酸＞12-(9-蒽基)硬脂酸＞16-(9-蒽基)硬脂酸。凝胶-液晶双层转变温度（Tc）的相应降低在荧光去极化和差示扫描量热数据中显示出相同的扰动顺序。将蒽基实体定位在双层的中心似乎能提供最小的扰动。
