高浓度的重组鼠干扰素-β可增强恙虫病东方体（原立克次体）吉利亚姆株在小鼠L929细胞中的生长。
High concentration of recombinant murine interferon-beta enhances the growth of Orientia (formerly Rickettsia) tsutsugamushi Gilliam in mouse L929 cells.
作者信息
Tasneen R, Yoshiie K, Ohkawa T, Oda H
机构信息
Department of Bacteriology, Faculty of Medicine, Kagoshima University, Japan.
出版信息
Acta Virol. 1997 Apr;41(2):77-82.
We studied the effect of recombinant murine interferons (rMuIFNs) on the growth of Orientia (formerly Rickettsia) tsutsugamushi Gilliam in mouse L929 cells. Rickettsial growth was measured by flow cytometry. rMUIFN-gamma inhibited the growth of O. tsutsugamushi at the concentrations of 100 i.u./ml and 1,000 i.u./ml in accord with previous reports. Relatively low concentrations (10 i.u./ml and 100 i.u./ml) of rMUIFN-beta also inhibited the growth of O. tsutsugamushi. On the other hand, high concentrations (1,000 i.u./ml and 10,000 i.u./ml) of rMuIFN-beta enhanced the growth of the rickettsia. This enhancement of rickettsial growth was blocked by anti-murine IFN-beta monoclonal antibody (MoAb). rMuIFN-beta also enhanced the growth of Rickettsia sibirica 246 in L929 cells to some extent.
我们研究了重组鼠干扰素（rMuIFNs）对鼠型恙虫病东方体（原立克次体）吉利亚姆株在小鼠L929细胞中生长的影响。通过流式细胞术检测立克次体的生长情况。与之前的报道一致，rMUIFN-γ在100国际单位/毫升和1000国际单位/毫升的浓度下抑制了恙虫病东方体的生长。相对较低浓度（10国际单位/毫升和100国际单位/毫升）的rMUIFN-β也抑制了恙虫病东方体的生长。另一方面，高浓度（1000国际单位/毫升和10000国际单位/毫升）的rMuIFN-β促进了立克次体的生长。这种立克次体生长的促进作用被抗鼠IFN-β单克隆抗体（MoAb）阻断。rMuIFN-β在一定程度上也促进了西伯利亚立克次体246株在L929细胞中的生长。
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