采用逆转录-聚合酶链反应对捷克共和国啤酒花品种中的苹果花叶病毒进行检测。
Screening of apple mosaic virus in hop cultivars in the Czech Republic by reverse transcription-polymerase chain reaction.
作者信息
Petrzik K, Svoboda P
机构信息
Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Ceské Budĕjovice, Czech Republic.
出版信息
Acta Virol. 1997 Apr;41(2):101-3.
PMID:9219641
Abstract
Thirteen cultivars of hop (Humulus lupulus L.) were tested by reverse transcription-polymerase chain reaction (RT-PCR) for the presence of apple mosaic virus (ApMV). The virus was detected in various amounts in all tested cultivars. Control hop clones derived from tissue cultures, treated by thermotherapy and maintained in greenhouse were virus-free. The procedure for sample preparation and RT-PCR of ApMV is described in detail.
摘要
通过逆转录聚合酶链反应（RT-PCR）对13个啤酒花（Humulus lupulus L.）品种进行了苹果花叶病毒（ApMV）检测。在所有测试品种中均检测到了不同含量的该病毒。源自组织培养、经温热疗法处理并保存在温室中的对照啤酒花克隆无病毒。详细描述了ApMV的样品制备和RT-PCR程序。
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