Cloning and in vitro expression of rat lecithin:cholesterol acyltransferase.

作者信息

Wang J, Gebre A K, Anderson R A, Parks J S

机构信息

Department of Comparative Medicine, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27157, USA.

出版信息

Biochim Biophys Acta. 1997 Jun 23;1346(3):207-11. doi: 10.1016/s0005-2760(97)00055-6.

Rat lecithin:cholesterol acyltransferase (LCAT) cDNA was obtained by reverse transcriptase/polymerase chain reaction amplification of rat liver total RNA. A consensus sequence was derived from four independent clones from two strains of rats. In vitro expression of rat LCAT cDNA in COS cells resulted in secreted enzyme protein with the same fatty acyl specificity for phospholipase A2 activity and cholesterol esterification as rat plasma LCAT, but different from that of recombinant or human plasma LCAT.