Fiscella R G, Moshifar M, Messick C R, Pendland S L, Chandler J W, Viana M
Department of Pharmacy Practice, University of Illinois at Chicago 60612, USA.
Cornea. 1997 Jul;16(4):447-9.
We wanted to determine whether topical polyhexamethylene biguanide (PHMB) 0.02% was effective in the treatment of experimental Fusarium keratomycosis in rabbits.
Fusarium solani keratomycosis was induced in the eyes of 12 New Zealand white rabbits. The rabbits were treated with PHMB 0.02% in one eye and placebo in the other eye for 6 days. The rabbits were evaluated in a masked fashion using a standardized system for clinical progression of the disease. Then the corneas were trephined and growth of F. solani in colony-forming units per milliliter (CFU/ml) determined.
Clinical evaluation demonstrated no significant mean difference (p > 0.10) in clinical scores between treated and control eyes on day 6 (0.583 +/- 2.503). There was a significant mean CFU difference (p = 0.06) between treated eyes and control eyes (182.5 +/- 314.44). Seven of 12 eyes (58%) in the PHMB group exhibited no growth, whereas two of 12 (17%) eyes reported no growth in the control group. One of 12 eyes (8%) reported > 100 CFU in the PHMB group, whereas seven of 12 eyes (58%) reported > 100 CFU in the control group.
PHMB 0.02% was effective in significantly reducing the fungal growth in our rabbit model of Fusarium keratomycosis. The future role of PHMB in the treatment of Fusarium keratitis needs to be further evaluated.
我们想要确定0.02%的局部聚六亚甲基双胍（PHMB）对兔实验性镰刀菌性角膜溃疡的治疗是否有效。
在12只新西兰白兔的眼睛中诱发茄病镰刀菌性角膜溃疡。兔子一只眼睛用0.02%的PHMB治疗，另一只眼睛用安慰剂治疗6天。使用标准化系统以盲法评估兔子疾病的临床进展。然后切取角膜，测定每毫升茄病镰刀菌的菌落形成单位（CFU/ml）的生长情况。
临床评估显示，在第6天，治疗眼和对照眼的临床评分无显著平均差异（p>0.10）（0.583±2.503）。治疗眼和对照眼之间的平均CFU有显著差异（p = 0.06）（182.5±314.44）。PHMB组12只眼中有7只（58%）未生长，而对照组12只眼中有2只（17%）未生长。PHMB组12只眼中有1只（8%）报告CFU>100，而对照组12只眼中有7只（58%）报告CFU>100。
0.02%的PHMB能有效显著减少我们兔镰刀菌性角膜溃疡模型中的真菌生长。PHMB在镰刀菌性角膜炎治疗中的未来作用需要进一步评估。
