Weng J, Mohan R R, Li Q, Wilson S E
Eye Institute, Cleveland Clinic Foundation, Ohio 44195, USA.
Cornea. 1997 Jul;16(4):465-71.
To determine whether interleukin 1 beta (IL-1 beta) messenger RNA (mRNA) and protein were expressed in corneal cells and to examine the effects of IL-1 alpha and IL-1 beta on the expression of hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF) mRNAs and proteins in corneal stromal fibroblasts.
IL-1 beta mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). IL-1 beta protein was detected by immunohistologic tests. Changes in the expression of HGF and KGF mRNAs and proteins in response to stimulation of cultured corneal stromal fibroblasts with IL-1 alpha and IL-1 beta were monitored by Northern and Western blotting, respectively.
IL-1 beta mRNA is expressed in human primary cultured corneal epithelial, stromal fibroblast, and endothelial cells. IL-1 beta protein was detected in epithelium and endothelium in fresh frozen human and rabbit corneal tissue. Little, if any, IL-1 beta was detected in the unwounded corneal stroma. IL-1 alpha and IL-1 beta at 10 ng/ml upregulated the levels of HGF and KGF mRNAs and proteins in cultured human corneal fibroblasts.
IL-1 alpha and IL-1 beta may serve as key modulators in an epithelial-stromal regulatory loop in the cornea. These data and previously published observations support the hypothesis that corneal epithelial wounding releases IL-1 alpha and IL-1 beta from epithelial cells; these cytokines in turn upregulate HGF and KGF mRNA and protein levels in keratocytes, and HGF and KGF released by the keratocytes modulate healing of the wounded corneal epithelial cells by regulating proliferation, motility, and differentiation.
确定白细胞介素1β（IL-1β）信使核糖核酸（mRNA）和蛋白是否在角膜细胞中表达，并检测IL-1α和IL-1β对角膜基质成纤维细胞中肝细胞生长因子（HGF）和角质形成细胞生长因子（KGF）mRNA及蛋白表达的影响。
采用逆转录聚合酶链反应（RT-PCR）检测IL-1β mRNA。通过免疫组织学检测法检测IL-1β蛋白。分别采用Northern印迹法和Western印迹法监测用IL-1α和IL-1β刺激培养的角膜基质成纤维细胞后HGF和KGF mRNA及蛋白表达的变化。
IL-1β mRNA在人原代培养的角膜上皮细胞、基质成纤维细胞和内皮细胞中表达。在新鲜冷冻的人及兔角膜组织的上皮和内皮中检测到IL-1β蛋白。在未受伤的角膜基质中几乎未检测到IL-1β（若有也极少）。10 ng/ml的IL-1α和IL-1β上调了培养的人角膜成纤维细胞中HGF和KGF mRNA及蛋白的水平。
IL-1α和IL-1β可能是角膜上皮-基质调节环路中的关键调节因子。这些数据及先前发表的观察结果支持以下假说：角膜上皮损伤会从上皮细胞释放IL-1α和IL-1β；这些细胞因子进而上调角膜细胞中HGF和KGF mRNA及蛋白水平，而角膜细胞释放的HGF和KGF通过调节增殖、迁移和分化来调节受伤角膜上皮细胞的愈合。
