Huang R R, Rapoport D, Schaeffer M T, Cascieri M A, Fong T M
Merck Research Laboratories, Rahway, NJ 07065, USA.
J Recept Signal Transduct Res. 1997 Jul;17(4):599-607. doi: 10.3109/10799899709039152.
We report the isolation of the genes encoding the beta 1 and beta 2 adrenergic receptors from dog genomic DNA. Sequence analysis of both genes revealed intronless open reading frames of 473 and 415 amino acid residues, receptively. Heterologous expression of both receptors in CHO cells indicated that both receptors are functionally similar to the human homologs. Comparing the dog beta 1 and beta 2 adrenergic receptors, the beta 1 receptor appears to bind to G proteins more tightly than the beta 2 receptor. Heterologously expressed receptors provide a convenient system for evaluating novel receptor agonists and antagonists.
我们报道了从犬基因组DNA中分离出编码β1和β2肾上腺素能受体的基因。对这两个基因的序列分析分别揭示了由473和415个氨基酸残基组成的无内含子开放阅读框。这两种受体在CHO细胞中的异源表达表明，它们在功能上与人源同源物相似。比较犬β1和β2肾上腺素能受体，β1受体似乎比β2受体与G蛋白的结合更紧密。异源表达的受体为评估新型受体激动剂和拮抗剂提供了一个便利的系统。
