Heterogeneity of steroid hormone receptor in adult rat lung cytosol.

Chromatography on cellulose DEAE-52 columns revealed that the glucocorticoid receptor for rat lung cytosol consisted of a component in the 0.001 M prewash, revealed with synthetic steroids and natural mineralocorticoids, a second component eluted with 0.04 M PO4, labelled with triamcinolone, dexamethasone, and a third moiety in the 0.06 M PO4 region, evident with natural glucocorticoids (corticosterone, cortisol) as well as mineralocorticoids (aldosterone, deoxycorticosterone). The third component coeluted with rat blood serum transcortin in double labelled experiments. Rate lung was devoid of another component in the 0.02 M PO4 found in rat liver supernate and of the mineralocorticoid receptor evident only in rat kidney. Chromatography on Sephadex G-200 columns revealed a shift of radioactivity from a higher to a lower molecular weight region in the presence of 0.4 M KCl. Collectively, these studies indicate the subunit nature of the lung receptor as evidenced in most tissues hitherto tested. Moreover, polymorphism within a given subunit component can not be revealed by competition alone, as attempted by others, but can be revealed under selected conditions of physical separation.