Levchenko L A, Raevskiĭ A V, Likhtenshteĭn G I, Sadkov A P, Pivovarova T S
Biokhimiia. 1977 Oct;42(10):1755-64.
The electron microscopy method has been used for studying the topography of the active centre of the nitrogenase in combination with the method of electron density labels. The active centre has been shown to place on the nitrogenase macromolecule asymmetrically. The non-heme iron atoms of the nitrogenase active centre have been regularly packed and located on the macromolecule as a quadrangular cluster closely to the surface of subunits. Two free sulfhydryl groups of the ATP-site of the nitrogenase active centre are in close proximity to the cluster. ATP-site of the nitrogenase active centre containing these groups is located on the Fe-enzyme, while the main part of the iron-containing cluster is located on the Mo-Fe-enzyme.
电子显微镜方法已与电子密度标记法相结合用于研究固氮酶活性中心的拓扑结构。已表明活性中心不对称地位于固氮酶大分子上。固氮酶活性中心的非血红素铁原子规则排列，并作为四边形簇位于大分子上，靠近亚基表面。固氮酶活性中心ATP位点的两个游离巯基与该簇紧密相邻。含有这些基团的固氮酶活性中心的ATP位点位于铁蛋白上，而含铁簇的主要部分位于钼铁蛋白上。
